香附四物汤对小鼠脾淋巴细胞增殖转化影响的效应物质基础研究

刘培; 段金廒; 洪敏; 赵金龙

您当前的位置：

首页 >

文章列表页 >

香附四物汤对小鼠脾淋巴细胞增殖转化影响的效应物质基础研究

更新时间：2024-01-04

- 香附四物汤对小鼠脾淋巴细胞增殖转化影响的效应物质基础研究
- Study on Mice Spleen Lymphocyte Proliferation and Transformation Effect and Chemical Constituents of Xiangfu Siwu Decoction
- 中国实验方剂学杂志 2012年18卷第23期页码：252-257
- 作者机构：
- 作者简介：
- 基金信息：
  
  江苏省高校自然科学基金重大基础研究项目(06KJA36022);南京中医药大学方剂重点学科项目(2011FJ006)
- DOI：
  中图分类号：
- 纸质出版日期：2012
- 稿件说明：
移动端阅览
刘培, 段金廒, 洪敏, 等. 香附四物汤对小鼠脾淋巴细胞增殖转化影响的效应物质基础研究[J]. 中国实验方剂学杂志, 2012,18(23):252-257.

LIU Pei, DUAN Jin-ao, HONG Min, et al. Study on Mice Spleen Lymphocyte Proliferation and Transformation Effect and Chemical Constituents of Xiangfu Siwu Decoction[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(23): 252-257.
刘培, 段金廒, 洪敏, 等. 香附四物汤对小鼠脾淋巴细胞增殖转化影响的效应物质基础研究[J]. 中国实验方剂学杂志, 2012,18(23):252-257. DOI：

LIU Pei, DUAN Jin-ao, HONG Min, et al. Study on Mice Spleen Lymphocyte Proliferation and Transformation Effect and Chemical Constituents of Xiangfu Siwu Decoction[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(23): 252-257. DOI：

摘要

目的: 评价香附四物汤及各分离化学部位对小鼠脾淋巴细胞增殖转化的影响。方法: 采用小鼠脾淋巴细胞增殖与转化实验方法

对香附四物汤及各分离化学部位进行免疫相关的生物效应评价;采用HPLC对效应显著部位的化学成分进行定性定量分析。结果: 香附四物汤、醇沉上清液部位及其经大孔吸附树脂吸附后30%乙醇洗脱部位具有明显促进小鼠脾淋巴细胞增殖与转化作用

其中

30%乙醇洗脱部位的生物效应显著;对30%效应部位进行定性定量分析表明其主要组成及其含量分别为芍药内酯苷5.973%

芍药苷5.902%

阿魏酸1.374%

洋川芎内酯I 0.014%。结论: 香附四物汤对小鼠脾淋巴细胞增殖转化影响的主要效应物质基础是含有芍药内酯苷、芍药苷、阿魏酸和洋川芎内酯I等的30%乙醇洗脱部位。

Abstract

Objective: To evaluate the mice spleen lymphocyte proliferation and transformation effect of Xiangfu Siwu decoction(XFSWD) and explore the bioactive constituents. Method: The samples were prepared by macroporous adsorptive resins. mice spleen lymphocyte proliferation and transformation models were adopted to evaluate the immune related activities of XFSWD and its different separated fractions in vitro. The chemical constituents in the most active fraction were identified and determined by HPLC. Result: By mice spleen lymphocyte proliferation and transformation experiments

XFSWD

XFSW-3 and XFSW-7 showed high effect. Four main compounds including albiflorin

peoniflorin

ferulaic acid

senkyunolide I in active fraction XFSW-7 were identified and quantified by HPLC-DAD. The contents were 5.973%

5.902%

1.374% and 0.014%

respectively. Conclusion: Albiflorin

peoniflorin

ferulaic acid

senkyunolide I were the potential active constituents. XFSWD had the common material basis.

关键词

Keywords

references

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

香附四物汤与四物汤对急性血瘀模型大鼠血液流变性及卵巢功能的影响

基于正/反向效应评价香附四物汤中主要药对的作用特点及对整方的贡献

香附四物汤对气滞血瘀证雌性大鼠神经-内分泌-免疫网络的调控与影响