Objective:HPLC chromatographic fingerprints for Shuangshan granules were developed in order to evaluate the internal quality. Method: HPLC analysis was performed at 25 ℃ by using a Diamonsil C18 column (4.6 mm×250 mm

5 μm)

with the gradient elute of mobile phase consisting of acetonitrile-0.1% glacial acetic acid at the flow rate of 1.0 mL·min-1. The UV detection was set at 340 nm. Result: Chlorogenic acid was used as the reference substance in the HPLC chromatographic fingerprint of Shuangshan Granules

which showed 13 common peaks and the similarity was 0.98 among 10 samples. Conclusion: This method was accurate

repeatable and useful for the quality control of Shuangshan granules.