HPLC测定全蚕粉中槲皮素和山奈素的含量

赵艳丽; 黄亦琦; 胡珊梅; 杨辉

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HPLC测定全蚕粉中槲皮素和山奈素的含量

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- HPLC测定全蚕粉中槲皮素和山奈素的含量
- Content Determination of Quercetin and Kaempferol in Silkworm Powder by RP-HPLC
- 中国实验方剂学杂志 2012年18卷第18期页码：95-97
- 作者机构：
- 作者简介：
- 基金信息：
  
  福建省自然科学基金项目(面上项目2011D016)
- DOI：
  中图分类号：
- 纸质出版日期：2012
- 稿件说明：
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赵艳丽, 黄亦琦, 胡珊梅, 等. HPLC测定全蚕粉中槲皮素和山奈素的含量[J]. 中国实验方剂学杂志, 2012,18(18):95-97.

ZHAO Yan-li, HUANG Yi-qi, HU Shan-mei, et al. Content Determination of Quercetin and Kaempferol in Silkworm Powder by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(18): 95-97.
赵艳丽, 黄亦琦, 胡珊梅, 等. HPLC测定全蚕粉中槲皮素和山奈素的含量[J]. 中国实验方剂学杂志, 2012,18(18):95-97. DOI：

ZHAO Yan-li, HUANG Yi-qi, HU Shan-mei, et al. Content Determination of Quercetin and Kaempferol in Silkworm Powder by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(18): 95-97. DOI：

摘要

目的: 建立全蚕粉中槲皮素、山奈素的含量测定方法。方法: 采用反相高效液相色谱法(RP-HPLC)

色谱柱为KromasiL-C18柱 (4.6 mm×250 mm

5 μm)

柱温为室温

流动相甲醇-0.4%磷酸(50:50)

流速1.0 mL ·min-1

检测波长360 nm

进样量10 μL。结果: 槲皮素和山奈素得到良好分离

分别在0.012 5~0.400 0 μg(r=0.999 8)

0.014 0~0.448 0 μg(r=0.999 9)呈良好的线性关系

平均回收率分别为97.53%(RSD 1.9%)

100.3%(RSD 1.0%)。结论: 该方法快速准确

可满足定量分析的需要。

Abstract

Objective: To establish assaying methods for the determination of quercetin and kaempferol in Silkworm Powder. Method: The content of quercetin and kaempferol was determined by RP-HPLC

using a KromasiL-C18 column (4.6 mm×250 mm

5 μm). The column temperature was fixed on room temperature. Methanol-0.4% phosphoric acid (50: 50) was used as mobile phase

flow-rate was 1.0 mL · min-1.The detecting wavelength was set at 360 nm. Result: The linear range were 0.012 5-0.4 μg (r=0.999 8) and 0.014-0.448 μg (r=0.999 9). The average recoveries were 97.53% and 100.3%

with the RSD of 1.9% and 1.0%. Conclusion: The method established is rapid and accurate

which could be used for quality control of Silkworm Powder.

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