Objective: To study the effect of Dongyao Wuwei Zhixie decoction on expressions of heat shock protein 70(HSP70)mRNA and nuclear factor kappa B p65 (NF-κB p65)in ulcerative colitis rats and its mechanisms. Method: Sixty SD rats were randomly divided into normal group

model group

Dongyao Wuwei Zhixie decoction low

middle and high dose groups

Mesalazine group

10 each group. 2

4

6-trinitrobenzene sulfonic acid (TNBS)/ethanol solution was administered by enema to make ulcerative colitis model.The activity index (DAI) and colonic mucosal injury degree were observed

NF-κB p65 was determined by immunohistochemical staining (SABC method) and colonic tissue HSP70 mRNA expression was detected real time fluorescent quantitative PCR (real-time PCR) 10 days later. Result: Dongyao Wuwei Zhixie decoction can significantly reduce the DAI sores and tissue damage score of the rats’ colon

increase the HSP70 mRNA level

and decrease the expressions of NF-κB p65 in rats. Conclusion: Dongyao Wuwei Zhixie Decoction in the treatment of UC mechanism may be associated with the enhancement of protective protein HSP70 mRNA expression level and inhibition of NF- κB p65 activation.