Objective:Identification and quality evaluation of complex traditional Chinese medicine preparation of Qinbaiqingfeinongsuo Pills were carried out by HPLC fingerprint analysis. Method: UV-ELSD fingerprint of Qinbaiqingfeinongsuo Pills was conducted with Zorbax column and non-linear elution with the mobile phase consisted of acetonitrile-1.5％glacial acetic acid and detective wavelength was set at 280 nm. Result: Components of Qinbaiqing feinongsuo Pills were separated completely in the chromatographic condition. The standard fingerprint consisted 10 co-possessing peaks. Conclusion: The established HPLC fingerprint represents the whole character of Qinbaiqingfeinongsuo Pills. The method is found to be simple and accurate which enhanced the specialty for control and assessment of the product quality.