Objective: To study the antiphlogistic and antibacterial effect of aqueous extract from Mussaenda pubescens. Method: Mice were divided into 5 groups randomly:normal saline (NS)group

positive group

high dose group

middle dose group and low dose group of aqueous extract from M. pubescens (33.6

16.8

8.4 g·kg-1). After 4 d by ig administration

dimethybenzene-induced mouse ear edema model was used to evaluate the anti-inflammation effect. Rats were divided into 5 groups randomly:normal saline (NS) group

positive group

high dose group

middle dose group and low dose group of aqueous extract from M.pubescens (24

12

6 g·kg-1). Using models of carrageenan-induced paw edema and cotton-peller-induced granuloma

anti-inflammation effect was evaluated. Bacteriostasis effect of aqueous extract from M.pubescens was investigated by the method of bacteriostatic test in vitro. Result: The middle and high dose of the extract could significantly relieve ear-swelling caused by dimethylbenzene (P<0.05).The middle and high dose of the extract could significantly inhibit paw swelling in rats within 2

3 hours (P<0.05).The granuloma quality was significantly reduced by the extract compared with the control group (P<0.05). The aqueous extract had antibacterial effects on Staphylococcus aureus

Escherichia coli

Pneumococcus

Streptococcus

Shigella

and the minimum inhibitory concentration(MIC) was 125

31.3

15.7

62.5

62.5 mg·kg-1

respectively. Conclusion: Aqueous extract from M. pubescens has significant anti-inflammatory and antibacterial effects.