Objective: To prepare Zhixue Zhentong powder

and establish its quality standard to control its therapeutic efficacy. Method: Total flavonoids from water extract of Lamiophlomis rotatawas removed by polyamide column

total iridoid glycoside was enriched by macroporous resin

Zhixue Zhentong powder was prepared with iridoid glycoside as raw material.TLC qualitative identification was adopted with 8-O- acetylshanzhiside methylester as reference substance; The content of total iridoid glycosides was determined by first derivative ultraviolet spectrophotometry;the content of shanzhiside methylester and 8-O- acetylshanzhiside methylester were determined by HPLC. Result: TLC spots of shanzhiside methylester and 8-O- acetylshanzhiside methylester were all clear. Total iridoid glycosides determination for this preparation was established

average recovery of this method was 104.40%

RSD 0.64%. HPLCfor determination of shanzhiside methylester and 8-O- acetylshanzhiside methylester in this preparation was established

average recoveries were 94.74%

100.59%

RSD were 2.27%

1.61%

respectivly. Conclusion: Optimized preparation technology of Zhixue Zhentong powder could be extended for use in industrial production;This established quality control method was accurate and reliable

it could evaluate quality of Zhixue Zhentong powder effectively.