Objective: To optimize extracting process of total alkaloids from Zanthoxylum nitidum by enzymatic hydrolysis assisted immersion. Method: With extraction rate of total alkaloids as index

effect of 5 factors on enzymatic technology was investigated by single factor test

including kind of enzyme

enzyme dosage

pH

temperature and time;extraction times

extraction time and the concentration of ethanol were chosen as factors

effects of 3 factors on immersion technology of total alkaloids from Z. nitidum was optimized by orthogonal test. Result: Optimized enzymatic hydrolysis process was as following:cellulase-substrate and pectinase-substrate of 1∶250

4 times buffer with pH 5

enzyme pretreating for 30 min at ambient temperature (30 ℃);Optimum immersion extracting technology was following:extracted 3 times with 60% ethanol of 12

4

3 times amount(hydrochloric acid of 5 g·L-1 )

at ambient temperature

2 h each time. Extraction rate of total alkaloids of 85.22% by this process. Conclusion: This optimized process was simple

efficient

energy saving and environmentally friendly

and it could provide experimental base for industrial production.