小飞蓬药材薄层鉴别与含量测定

邵帅; 严铭铭; 毕胜男; 万志强; 黄耀玲

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小飞蓬药材薄层鉴别与含量测定

更新时间：2024-01-04

- 小飞蓬药材薄层鉴别与含量测定
- Identification and Determination of
- 中国实验方剂学杂志 2012年18卷第24期页码：101-103
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- 基金信息：
  
  国家重大新药创制专项(2009ZX09103-323)
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- 纸质出版日期：2012
- 稿件说明：
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邵帅, 严铭铭, 毕胜男, 等. 小飞蓬药材薄层鉴别与含量测定[J]. 中国实验方剂学杂志, 2012,18(24):101-103.

SHAO Shuai, YAN Ming-ming, BI Sheng-nan, et al. Identification and Determination of [J]. Chinese journal of experimental traditional medical formulae, 2012, 18(24): 101-103.
邵帅, 严铭铭, 毕胜男, 等. 小飞蓬药材薄层鉴别与含量测定[J]. 中国实验方剂学杂志, 2012,18(24):101-103. DOI：

SHAO Shuai, YAN Ming-ming, BI Sheng-nan, et al. Identification and Determination of [J]. Chinese journal of experimental traditional medical formulae, 2012, 18(24): 101-103. DOI：

摘要

目的: 探讨小飞蓬药材定性、定量检测方法。方法: 采用TLC鉴别小飞蓬中的槲皮素

HPLC测定小飞蓬中槲皮素的含量。结果: TLC以正己烷-乙酸丁酯-甲酸(6∶5∶1) 为展开系统

可以清晰地检测槲皮素; HPLC采用日本岛津Shimadzu C18(4.6 mm×250 mm

5 μm)色谱柱

流动相-甲醇-0.4%磷酸(47∶53)

检测波长257 nm

流速1 mL·min-1

柱温30 ℃

槲皮素在0.504~1.134 μg呈良好线性关系

平均回收率分别为97.97%%

RSD分别为1.2 %。结论: 该法可作为小飞蓬药材的定性、定量检测方法。

Abstract

Objective:To discuss detection method of the qualitative and quantitative of Erigeron canawdensis. Method: TLC was used to determine quercetin of E. canawdensis. The content of quercetin was determined by HPLC. Result: n-Hexane-butyl acetate-formic (6∶5∶1) is used as TLC deployment system. It may clearly detect quercetin; Shimadzu C18 (4.6 mm×250 mm

5 μm) column was used in HPLC with mobile phase methanol-0.4% phosphoric acid (47∶53). The detection wavelength was set at 257 nm and the flow rate was 1 mL·min-1; the column temperature was at 30 ℃.The linear range of quercetin were respectively 0.504-1.134 μg

and their average recovery were respectively 97.97%

RSD were respectively 1.2%. Conclusion: This method is used as detection method of the qualitative and quantitative of E. canawdensis.

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