Objective:To observe the effect of Fengshiqing (FSQ) conlaining serum on the expression of osteoprotegerin(OPG)

receptor activator for nuclear factor-κB ligand(RANKL)

tumor necrosis factor-α(TNF-α) and interleukin-17 (IL-17) in human fibroblast-like synoviocytes of rheumatoid arthritis(RA-HFLS). Method: RA-HFLS were cultured with different volume ration of FSQ in serum. Cell proliferation was evaluated by MTS assay.The expression of OPG

IL-17 and TNF-α in cell culture supernatant was examined by ELISA

and expression of RANKL was detected by Western blot analysis. Result: FSQ containing serum could obviously decrease the RA-HFLS viability in a dose-dependent style. The RA-HFLS could secret certain level of OPG

and FSQ containing surum could promote the secretion of OPG with a dose-dependent style. FSQ containing surum also suppressed the expression of RANKL

IL-17 and TNF-α. Conclusion: FSQ has an notable inhibiting effect on proliferation of RA-HFLS

increasing the level of OPG

and suppressing expression of RANKL

IL-17 and TNF-α

which may be a part of the mechanism of FSQ for rheumatoid arthritis.