Objective:To study the effective fraction and compounds from Honeysuckle on inhibition of bacterial biofilms. Method: The micro-plate method was used as bioassay-guided and compounds were isolated from effective fraction by repeated silica gel column chromatography. Research their effect of bacterial biofilms. Result: The ethanol extract fraction was active site and 15 compounds were obtained from it. Soya-cerebrosideⅡ ( 1 )

5-o-cafffeoyl quinic acid butyl ester ( 2 )

ginnol ( 3 )

sweroside ( 4 )

7-epi-vogeloside ( 5 )

stearyl ( 6 )

octacoylalcohol ( 7 )

protocatechuic aldehyde ( 8 )

protocatechuic ( 9 )

cinnamic acid ( 10 )

caffeic acid ( 11 )

ursolic acid ( 12 )

quercetin ( 13 )

oleanolic acid ( 14 )

ferulic acid ( 15 ). When the concentration was 1 g·L-1

the inhibition rates of compound 4

5

12 were 36.16%

37.16% and 46.18% separately. Conclusion: Compound 1 isolated from Honeysuckle for the first time. The compound 4

5

12 had certain inhibition on bacterial biofilms of Escherichia coli biofilms.