Objective: To explore purification technology of total phenol from seed of Forsythia suspense by macroporous adsorption resin. Method: With adsorption capacity and desorption ratio of total phenol from seed of F. suspense as indexes

optimum resin model was screened from AB-8

CAD-40

HPD-300 and D101 macroporous resin; With yield of total phenol from seed of F. suspense as index

effect of four factors on purification technology for total phenol from seed of F. suspense was investigated by single factor test

such as the amount of resin and water

kind and amount of elution solvent;With yield of total phenol from seed of F. suspense as index

adsorption flow rate

elution flow rate and resin diameter-high ratio were selected as factors

purification technology of total phenol from seed of F. suspense was optimized by orthogonal test. Result: Experiment result showed that AB-8 resin possessed optimal purification effect

its optimized purification technology was as follows:resin-crude drug 2: 1

diameter-high ratio 1: 5

adsorption flow rate 0.25 BV·h-1

washed by 5 BV water to impurity

eluted with 2 BV 80% ethanol

elution flow rate 0.25 BV·h-1.Under these technology conditions

purity of total phenol from seed of F. suspense was up to 65.56%. Conclusion: Optimized purification technology of total phenol was stable and feasible

it provided evidence for industrial production of total phenol from seed of F. suspense.