Objective: To establish an effective TLC and HPLC method for qualitative and quantitative analysis of salviaflaside in P. vulgariswhich can be used to be one of the quality control of P. vulgaris. Method: Salviaflaside was identified by TLC in P. vulgaris and different parts from P. vulgaris;salviaflaside was determined by RP-HPLC. Chromatography conditions were Agilent Eclipse XDB-C18(4.6 mm×250 mm

5μm) column with mobile phase of acetonitrile-1.0% acetic acid (16: 84)

UV detection wavelength was set at 319 nm and the column temperature was set at 30℃ with the flow rate of 1.0 mL·min-1; the sample injection was 10 μL. Result: The TLC sports developed were fairly clear only in flower spike with no interference; good linearity of salviaflaside was obtained in the range of 0.032 667-0.653 34 μg (r=0.999 9). The recovery of salviaflaside was 99.18% and RSD was 0.25%. The content of salviaflaside was more than 0.1‰. Conclusion: Salviaflaside was characteristic compound in flower spike of P. vulgaris. The method is simple

reliable and accurate

which can be applied as the quantity control method of P. vulgaris.