正交试验优选丁香抑马拉色菌活性物质提取工艺

宋杰; 董银卯; 孟宏

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正交试验优选丁香抑马拉色菌活性物质提取工艺

更新时间：2024-01-04

- 正交试验优选丁香抑马拉色菌活性物质提取工艺
- Optimization of Extraction Technology for Anti-Malassezia Furfur Active Substances from Eugenia caryophyllata by Orthogonal Test
- 中国实验方剂学杂志 2012年18卷第22期页码：68-71
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- 纸质出版日期：2012
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宋杰, 董银卯, 孟宏. 正交试验优选丁香抑马拉色菌活性物质提取工艺[J]. 中国实验方剂学杂志, 2012,18(22):68-71.

SONG Jie, DONG Yin-mao, MENG Hong. Optimization of Extraction Technology for Anti-Malassezia Furfur Active Substances from Eugenia caryophyllata by Orthogonal Test[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(22): 68-71.
宋杰, 董银卯, 孟宏. 正交试验优选丁香抑马拉色菌活性物质提取工艺[J]. 中国实验方剂学杂志, 2012,18(22):68-71. DOI：

SONG Jie, DONG Yin-mao, MENG Hong. Optimization of Extraction Technology for Anti-Malassezia Furfur Active Substances from Eugenia caryophyllata by Orthogonal Test[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(22): 68-71. DOI：

摘要

目的: 优选丁香中抑菌成分的提取工艺。方法: 以糠秕马拉色菌为供试菌

通过管碟法测试抑菌效果

通过单因素试验和正交试验确定丁香提取物的最优提取工艺。结果: 丁香最优提取工艺为70%乙醇回流提取

料液比1∶25

提取时间2 h

提取温度65 ℃。丁香醇提物对糠秕马拉色菌的抑菌圈直径为(23.08±0.46)mm

抑菌活力为高度敏感。结论: 优选的提取工艺稳定可行。

Abstract

Objective:To optimize of extraction technology of antibacterial substances from Eugenia caryophyllata. Method: With malassezia furfur as test bacteria

antibacterial effect was tested by cup-plate method

extraction technology of extract from E. caryophyllata was optimized by single factor test and orthogonal test. Result: Optimum extraction technology was as following:refluxing extracted 2 h with 25 times the amount of 70% ethanol at 65 ℃.diameter of inhibition zone was (23.08±0.46) mm

antibacterial activity was hypersensitive. Conclusion: This optimized technology was stable and feasible.

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