绞股蓝总皂苷对人肝癌细胞Bel-7402增殖与凋亡的影响

李晓龙; 吴育朗; 林子群; 莫淼; 梁雅杏; 潘越; 刘诗良

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绞股蓝总皂苷对人肝癌细胞Bel-7402增殖与凋亡的影响

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- 绞股蓝总皂苷对人肝癌细胞Bel-7402增殖与凋亡的影响
- Proliferation and Apoptosis Effects of Gypenosides on Human Hepatocellular Carcinoma Bel-7402 Cells
- 中国实验方剂学杂志 2012年18卷第19期页码：238-241
- 作者机构：
- 作者简介：
- 基金信息：
  
  广西医科大学青年科学基金项目(02602211010);实验室开放课题(02608211024)
- DOI：
  中图分类号：
- 纸质出版日期：2012
- 稿件说明：
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李晓龙, 吴育朗, 林子群, 等. 绞股蓝总皂苷对人肝癌细胞Bel-7402增殖与凋亡的影响[J]. 中国实验方剂学杂志, 2012,18(19):238-241.

LI Xiao-long, WU Yu-lang, LIN Zi-qun, et al. Proliferation and Apoptosis Effects of Gypenosides on Human Hepatocellular Carcinoma Bel-7402 Cells[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(19): 238-241.
李晓龙, 吴育朗, 林子群, 等. 绞股蓝总皂苷对人肝癌细胞Bel-7402增殖与凋亡的影响[J]. 中国实验方剂学杂志, 2012,18(19):238-241. DOI：

LI Xiao-long, WU Yu-lang, LIN Zi-qun, et al. Proliferation and Apoptosis Effects of Gypenosides on Human Hepatocellular Carcinoma Bel-7402 Cells[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(19): 238-241. DOI：

摘要

目的:探讨绞股蓝总皂苷(gypenosides

Gyp)对人肝癌细胞Bel-7402增殖与凋亡的影响。方法:采用MTT法检测Gyp对体外培养的人肝癌细胞Bel-7402的增殖抑制作用;单细胞凝胶电泳法检测Gyp对人肝癌细胞Bel-7402DNA损伤的影响;RT-PCR技术从分子水平上检测Gyp对人肝癌细胞Bel-7402的诱导凋亡作用机制。结果:100~400μg.mL-1的Gyp对人肝癌细胞Bel-7402增殖生长有抑制作用

Gyp(182μg·mL-1)作用4

24h后

单细胞凝胶电泳观测到DNA损伤

12h时DNA损伤最大

达到(81.15±14.23)%

且有时间依赖性;Gyp组细胞caspase-8大量表达

对照组细胞无表达。结论:Gyp能抑制人肝癌细胞Bel-7402增殖且促进其凋亡

其作用机制与DNA损伤以及caspase-8的大量表达有关。

Abstract

Objective:To investigate the suppressive effect of gypenosides(Gyp) on human hepatocellular carcinoma Bel-7402 cells. Method:The growth inhibitation of human hepatocellular carcinoma Bel-7402 cells was detected by MTT assay. The DNA damage of human hepatocellular carcinoma Bel-7402 cells was detected by single cell gel electrophoresis; RT-PCR was utilized to investigate the apoptosis of human hepatocellular carcinoma Bel-7402 cells and its relationship with caspase-8 from molecule level. Result:Gyp (100-400 μg·mL-1) inhibied the growth of human hepatocellular carcinoma Bel-7402 cells. DNA damage was detected after Gyp (182 μg·mL-1) treated for 4

24 h. The change reached maximum at 12 h(81.15±14.23)% and with time-dependent. caspase-8 expressed strongly in Gyp group and nothing in control. Conclusion:Gyp has effects on cell viability

it could induce human hepatocellular carcinoma Bel-7402 cells apoptosis

DNA damage and caspase-8 expression.

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