Objective: To investigate the influence of Sheng-huang mixtrue on the apoptosis related proteins expression of Bcl-assosiated x protein(Bax)

B-cell leukemia/lymphoma 2-like proteins(Bcl-2) and Aspartate-specific cysteinyl proteinase(Caspase-3) in myocardial ischemia-reperfusion injury rats. Method: SD rats(n=48) were divided randomly into six groups: normal control group

model group

positive control group

low-dose

medium-dose and high-dose Sheng-huang mixtrue groups(n=8). After the drug was administered to the six groups seven days

the Myocardial Ischemia-reperfusion injury in rats was established. TUNEL was used to detect the myocardial apoptosis index

The protein expressions of Bax

Bcl-2 and Caspase-3 were assayed by western blotting. Result: In comparison of the five other groups with model group

Sheng-huang mixture can decrease the myocardial apoptosis index and the protein expressions of Bax and Caspase-3

but increase the protein expressions of Bcl-2 in rats and the ratio of Bcl-2/Bax. Among which high dose Sheng-huang mixtrue group decreased much more than other groups. Conclusion: Sheng-huang mixture has the protective effects on myocardial ischemia-reperfusion injury in rats

which can inhibit the myocardial apoptosis of myocardial ischemia-reperfusion injury by increasing the protein expressions of Bcl-2 and reducing the protein expressions of Bax and Caspase-3.