甜叶菊渣中总黄酮的提取工艺优选

史高峰; 王红玉; 王国英; 宫建辉; 王振菊

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甜叶菊渣中总黄酮的提取工艺优选

更新时间：2024-01-04

- 甜叶菊渣中总黄酮的提取工艺优选
- Optimization of Extraction Process for Total Flavonoids from Residues of
- 中国实验方剂学杂志 2012年18卷第22期页码：62-64
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- 纸质出版日期：2012
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史高峰, 王红玉, 王国英, 等. 甜叶菊渣中总黄酮的提取工艺优选[J]. 中国实验方剂学杂志, 2012,18(22):62-64.

SHI Gao-feng, WANG Hong-yu, WANG Guo-ying, et al. Optimization of Extraction Process for Total Flavonoids from Residues of [J]. Chinese journal of experimental traditional medical formulae, 2012, 18(22): 62-64.
史高峰, 王红玉, 王国英, 等. 甜叶菊渣中总黄酮的提取工艺优选[J]. 中国实验方剂学杂志, 2012,18(22):62-64. DOI：

SHI Gao-feng, WANG Hong-yu, WANG Guo-ying, et al. Optimization of Extraction Process for Total Flavonoids from Residues of [J]. Chinese journal of experimental traditional medical formulae, 2012, 18(22): 62-64. DOI：

摘要

目的: 优选甜叶菊渣中总黄酮提取工艺条件。方法: 以总黄酮得率为指标

采用单因素试验考察乙醇体积分数、提取时间、料液比、提取温度及回流次数5个影响因素对甜叶菊渣中总黄酮得率的影响;在单因素试验基础上

选取乙醇体积分数、提取时间、料液比、提取温度为考察因素

采用正交设计法优化甜叶菊渣中总黄酮的提取工艺条件。结果: 各因素对甜叶菊渣中总黄酮得率的影响顺序为料液比＞提取时间＞乙醇体积分数＞提取温度。最佳提取工艺条件为乙醇体积分数80%

提取时间2.5 h

料液比1∶20

提取温度80 ℃

回流3次

总黄酮得率4.21%。结论: 该优选工艺简单易行、成本低廉、安全可靠

有利于甜叶菊资源的合理利用。

Abstract

Objective:To optimize extraction technology conditions of total flavonoids from residues of Stevia rebaudiana. Method: With yield of total flavonoids as index

effect of the concentration of ethanol

extraction time

solid-liquid ratio

extraction temperature and reflux extraction times on yield of total flavonoids from S. rebaudiana were studied by single-factor test;Basis on single factor test

the concentration of ethanol

extraction time

solid-liquid ratio

extraction temperature were chosen as factors

extraction technology of total flavonoids from residues of S. rebaudiana was optimized by orthogonal test. Result: Influence of various factors on yield of total flavonoids were in order of solid-liquid ratio>extraction time>the concentration of ethanol>extraction temperature. Optimum extraction technology was:reflux extracted 3 times with 20 times the amount of 80% ethanol at 80 ℃

extraction time of 2.5 h

yield of total flavonoids of 4.21%. Conclusion: This optimized technology was simple

reliable and safe with low cost

and it benefited to rational utilization of S. rebaudiana resources.

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