HPLC对市售瓜蒌中5-羟甲基糠醛的含量测定

孙文; 巢志茂; 王淳; 吴晓毅; 谭志高

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HPLC对市售瓜蒌中5-羟甲基糠醛的含量测定

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- HPLC对市售瓜蒌中5-羟甲基糠醛的含量测定
- Determination of 5-Hydroxmethylfurfural in Commercial Trichosanthis Fructus by HPLC
- 中国实验方剂学杂志 2012年18卷第18期页码：73-76
- 作者机构：
- 作者简介：
- 基金信息：
  
  2011年中医药行业科研专项(201107009)
- DOI：
  中图分类号：
- 纸质出版日期：2012
- 稿件说明：
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孙文, 巢志茂, 王淳, 等. HPLC对市售瓜蒌中5-羟甲基糠醛的含量测定[J]. 中国实验方剂学杂志, 2012,18(18):73-76.

SUN Wen, CHAO Zhi-mao, WANG Chun, et al. Determination of 5-Hydroxmethylfurfural in Commercial Trichosanthis Fructus by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(18): 73-76.
孙文, 巢志茂, 王淳, 等. HPLC对市售瓜蒌中5-羟甲基糠醛的含量测定[J]. 中国实验方剂学杂志, 2012,18(18):73-76. DOI：

SUN Wen, CHAO Zhi-mao, WANG Chun, et al. Determination of 5-Hydroxmethylfurfural in Commercial Trichosanthis Fructus by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(18): 73-76. DOI：

摘要

目的: 建立常用中药瓜蒌中5-羟甲基糠醛(5-HMF)的高效液相色谱(HPLC)测定方法。方法: 采用HPLC法

ODS色谱柱(4.6 mm×250 mm

5 μm)

甲醇-水(15:85)为流动相

流速0.8 mL ·min-1

检测波长284 nm

柱温30℃。结果: 建立了瓜蒌饮片中5-HMF的HPLC测定方法

18批市售瓜蒌样品中5-HMF的含量范围为11.10~193.6 μg ·g-1。结论: 各地的商品瓜蒌中5-HMF的含量差异较大

该方法可作为评价瓜蒌饮片质量的指标之一。

Abstract

Objective: To establish an HPLC method for content determination of 5-hydroxymethylfurfural (5-HMF) in commercial Trichosanthis Fructus

a commonly used Chinese material medica. Method: The HPLC analysis was performed on an ODS column (4.6 mm×250 mm

5 μm) and the mobile phase consisted of methanol-water (15: 85) at a flow rate of 0.8 mL · min-1. The detective wavelength was set at 284 nm and the column temperature was maiutain at 30℃. Result: The HPLC method for content determination of 5-HMF in Trichosanthis Fructus was established. 5-HMF was detected in 18 batches of commercial Trichosanthis Fructus

the content of which were in the range of 11.10-193.6 μg ·g-1. Conclusion: The content of 5-HMF in commercial Trichosanthis Fructus is quite different. This method is suitable for the quality control of Trichosanthis Fructus.

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