Objective:To verify whether the micro ribonucleic acid (miRNA) in Salvia miltiorrhiza and Rehmannia glutinosa

and their miRNA could enter human body blood through the digestive tract after oral adninistration. Method:At room temperature of 25℃

based on published miRNA sequences of S. miltiorrhiza (4) and R. glutinosa (6)

10 DIG-labeled antisense probes were synthesized. The total RNAs from S. miltiorrhiza and R. glutinosa were extracted with Plant Micro RNA extraction kit

and the total RNAs from human blood were extracted with blood miRNA extraction kit

followed the steps provided by the kit

and then loaded onto 15% denaturing polyacrylamide gel and hybridized with the appropriate DIG-labeled probes. Result:Northern blotting detected miR156 and miR157 in S. miltiorrhiza

and all the 6 miRNAs of miR5140

miR5139

miR 5138

miR 5137

miR 5142 and miR 5141 in R. glutinosa

and 3 miRNAs(miR5140

miR5137 and miR5141) in the test group which tested with R. glutinosa. Conclusion:These results present the evidence of miRNA in S. miltiorrhiza and R. glutinosa

and the miRNAs of miR5140

miR5137 and miR5141 from R. glutinosa could enter human body blood through the digestive tract after water frying. Further studies on the functions of these miRNAs may offer new insights and may lead to novel approaches to the development of Chinese traditional medicine.