HPLC测定板蓝根及其制剂中水杨酸、苯甲酸的含量

年四辉; 李萍; 刘丽敏; 李丽华

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HPLC测定板蓝根及其制剂中水杨酸、苯甲酸的含量

更新时间：2024-01-04

- HPLC测定板蓝根及其制剂中水杨酸、苯甲酸的含量
- Determination of Salicylic Acid and Benzoic Acid in Radix Isatidis and its Preparations by HPLC
- 中国实验方剂学杂志 2012年18卷第21期页码：92-95
- 作者机构：
- 作者简介：
- 基金信息：
  
  皖南医学院中青年科研基金(WK200812);安徽省教育厅项目(KJ2009B104Z);芜湖市科技局重点项目(2009卫1-5)
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- 纸质出版日期：2012
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年四辉, 李萍, 刘丽敏, 等. HPLC测定板蓝根及其制剂中水杨酸、苯甲酸的含量[J]. 中国实验方剂学杂志, 2012,18(21):92-95.

NIAN Si-hui, LI Ping, LIU Li-min, et al. Determination of Salicylic Acid and Benzoic Acid in Radix Isatidis and its Preparations by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(21): 92-95.
年四辉, 李萍, 刘丽敏, 等. HPLC测定板蓝根及其制剂中水杨酸、苯甲酸的含量[J]. 中国实验方剂学杂志, 2012,18(21):92-95. DOI：

NIAN Si-hui, LI Ping, LIU Li-min, et al. Determination of Salicylic Acid and Benzoic Acid in Radix Isatidis and its Preparations by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(21): 92-95. DOI：

摘要

目的: 建立高效液相色谱法测定板蓝根药材及其制剂中水杨酸、苯甲酸含量方法。方法: 以苯甲酸、水杨酸为对照品

采用Lichrospher C18色谱柱(4.6 mm×250 mm

5.0 μm)

流动相甲醇-0.1%的磷酸溶液(42:58)

流速1 mL ·min-1

检测波长230 nm

测定板蓝根药材及其制剂中水杨酸、苯甲酸含量。结果: 水杨酸在4.36~21.8 mg ·L-1与峰面积线性关系良好(r=0.999 9)

苯甲酸在4.04~20.2 mg ·L-1内与峰面积线性关系良好(r=0.999 7);水杨酸平均加样回收率为98.45%

RSD 1.26% (n=9)

苯甲酸平均加样回收率为97.90%

RSD 1.36% (n=9)。结论: 该方法简便、准确、重复性好

可用于板蓝根药材及其制剂中苯甲酸、水杨酸的含量测定。

Abstract

Objective: To determine the content of salicylic acid and benzoic acid in Radix Isatidis and its preparations by HPLC. Method: The content of salicylic acid and benzoic acid in Radix Isatidis and its preparations was determined by HPLC based on a Lichrospher C18 column(4.6 mm×250 mm

5 um)with a gradient elution solvent system composed of methanol -0.1%H3PO4 water(42: 58)solution.The flow rate was 1.0 mL·min-1

and the detection wavelength was set at 230 nm. Result: Salicylic acid had good linearity in the range of 4.36-21.8 mg·L-1

and the average recovery rate was 98.45% with RSD of 1.26% (n=9)

while benzoic acid had good linearity in the range of 4.04-20.2 mg·L-1

and the average recovery rate was 97.90% with RSD of 1.36% (n=9). Conclusion: The method is simple

stable and reliable

which can be used to determine salicylic acid and benzoic acid in Radix Isatids and its preparations.

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