Objective: To establish a method for the determination of gallic acid

berberine hydrochloride

aloe emodin

rhein

emodin

chrysophanol and physcion in Shuanghuangqudu tablets. Method: The HPLC system consisted of a ZORBAX SB-Pheny Dimensions column(4.6 mm×15 cm

5 μm)

methanol-0.1% phosphoric acid as mobile phase (using gradient) with flowing rate 1.0 mL·min-1 and detected at 310 nm. Result: The linear ranges of gallic acid

berberine hydrochloride

aloe emodin

rhein

emodin

chrysophanol and physcion were 0.12-2.4 μg (r=0.9999)

0.11-2.2 μg (r=0.9999)

0.015-0.3 μg (r=0.9998)

0.02-0.4 μg (r=0.9998)

0.008-0.16 μg (r=0.9999)

0.013-0.26 μg (r=0.9998)

0.006-0.12 μg (r=0.9999)

respectively

and the average recoveries (n=9) were 100.97%

100.20%

99.01%

99.76%

101.10%

101.97%

100.56%

respectively. Conclusion: The method is simple and repeatable

which can be applied to determine seven ingriedients at the same test condition in Shuanghuangqudu tablets.