Objective:To study the effects of gingerol on the contents of liver microsomal cytochrome P450 and the activities of CYP2E2 and CYP3A in mice. Method: Gingerol(200

100

50 mg·kg-1·d-1) were ig given to male mice for 5 days

after the liver microsomes were prepared by calcium isolation

the effects of gingerol on the contents of mice liver heavy

microsomal protein

CYP450 and CYPb5 were determined and the activities of CYP2E2 and CYP3A were tested by using erythromycin and aminopyrine. Result: The gingerol had no effects on the contents of liver heavy and microsomal protein in mice treated with gingerol(200

100

50 mg·kg-1·d-1) but could markedly decrease and increase the contents of CYP450 and CYPb5

respectively(P<0.01); the activity of CYP2E1 was inhibited in mice treated with gingerol(200

100

50 mg·kg-1·d-1) (P<0.05) and the effect of inhibition became stronger with the dose increasing of gingerol. The activity of CYP3A was inhibited in mice treated with gingerol(200 mg·kg-1·d-1)

(P<0.05). Conclusion: Gingerol has the effects of inhibiting the content of CYP450 and the activities of CYP2E1 and CYP3A

the degree of inhibition is related to dose.