Objective:To investigate the effect of total saponins of astragalus (TSA) on ferroportin 1 in rats with anemia of ahronic disease (ACD) induced by complete Freund's adjuvant. Method:Complete Freund's adjuvant was intradermally injected into foot pad of rats to duplicate ACD model. Then TSA (different concentration

respectively 50

16

5 mg·kg-1) was given for 7 days by peritoneum. Erythropoietin was used as the positive control. Interleukin-6(IL-6)in serum was detected with (Enzyme linked immunoassay

ELISA) and the Fpn1 mRNA (in spleen

liver and duodenum) and Hamp mRNA (in liver) were detected with Quantitative real-time Polymerase Chain Reaction (qPCR). Result:IL-6 in serum

Fpn1 mRNA in liver and duodenum

Hamp mRNA in liver were increased obviously (P＜0.05)

but there wasn't any change in Fpn1 mRNA in spleen. Compared with model group

IL-6 were decreased significantly both by high and middle dose (P＜0.05)

but indistinctively by low dose. Fpn1 mRNA in liver and duodenum

Hamp mRNA in liver were decreased significantly and Fpn1 mRNA in spleen was increased in all TSA groups (P＜0.05). Otherwise

the effect of TSA on above-mentioned indexes was prior to positive drug (P＜0.05). Conclusion:There was obvious increasing of Fpn1 in liver and duodenum in ACD rats

but almost no change in spleen. Fpn1 decreasing corresponding to hepcidin increasing were not found in this experiment. TSA was found to have different effect to Fpn in different tissue.