LIU Chang-song, ZHANG Gui-jun, ZHANG Zhi-yuan, et al. Analysis of Water-soluble Polysaccharide Components from Herba Lysimachiae Foenum-graeti by GC-MS[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(19): 129-131.
LIU Chang-song, ZHANG Gui-jun, ZHANG Zhi-yuan, et al. Analysis of Water-soluble Polysaccharide Components from Herba Lysimachiae Foenum-graeti by GC-MS[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(19): 129-131.DOI:
Objective:Identifying the water-soluble polysaccharide components of Herba Lysimachiae Foenum-graeti
to establish the quality of specificity evaluation index and Chinese medicine's active components alignment
which laid the foundation of new drug development. Method:The preparation of polysaccharides was extracted by the methods of water extracting-alcohol precipitating
then Sevag method sinking in protein
H2O2 bleaching and the use of dialysis method refined. The derivative change of the refined polysaccharide was analyzes by GC-MS. Result:The polysaccharide of Herba Lysimachiae Foenum-graeti is composed of rhamnitol
arabinose
ribose
glucose
and xylose
the proportion of it is 1.03:9.17:4.74:18.95:17.11. Conclusion:GC-MS could rapidly ascertain the water-soluble polysaccharide components of Herba Lysimachiae Foenum-graeti. The water-soluble polysaccharide of Herba Lysimachiae Foenum-graeti can be used as a component of the quality evaluation index and drug components of the new drug research based material.