Objective: To establish an HPLC-ELSD method for the simultaneous determination of the content of chikusetsusaponin Ⅳa and 3β -([[BFQ]O-β-D-glucopyranuronosyl]oxy)-olean-12-ene-28-olc acid in the fruits of Acanthopanax gracilistylus. Method: The RP-HPLC-ELSD method was used. Chromatographic conditions:AlltimaTM-C18 chromatographic column (4.6 mm×250 mm

5 μm);acetonitrile(A)-0.5% acetic acid water(B) as mobile phase;column temperature at 35℃. ELSD detector conditions:drift tube temperature: 106℃;carrier gas(nitrogen) flow rate: 2.9 L · min-1;amplification factor:1;impactor:close. Result: The calibration curves showed good linear relationship in the range from 0.535 to 8.560 μg (r=0.996 5) for chikusetsusaponin Ⅳa and 0.515 to 8.240 μg (r=0.998 7) for 3β-( oxy)-olean-12-ene-28-olc acid. The average recovery rates(n=6) were 98.39% and 98.97%

and RSDs were 1.50% and 1.59%. Conclusion: This method is accurate

sensitive and can be used to determine the content of chikusetsusaponin Ⅳa and 3β-( oxy)-olean-12-ene-28-olc acid in the fruits of Acanthopanax gracilistylus.