ZHANG Ming, CHEN Hua-guo, ZHAO Chao, et al. Determination of Content of Total Flavonoids from [J]. Chinese journal of experimental traditional medical formulae, 2012, 18(18): 77-80.
ZHANG Ming, CHEN Hua-guo, ZHAO Chao, et al. Determination of Content of Total Flavonoids from [J]. Chinese journal of experimental traditional medical formulae, 2012, 18(18): 77-80.DOI:
Objective: To establish a method for determination of total flavonoids in Polygonum perfoliatum. Method: Total flavoaoids were determined by UV-Vis spectrophotometer with rutiu as reference substance and 0.1 mol · L-1 AlCl3
1 mol · L-1 NaAc as chromogenic agent; the detection wavelength was at 413 nm. Result: A good linear relationship was achieved over the concentration range of 6.24-21.84 μg(r =0.999 8) for Rutin. The regression equation of total flavonoids was Y=38.183X-0.001
with an average recovery of 97.4%
RSD 2.2%. Conclusion: The method is simple and stable
and it is suitable for the content determination of total flavonoids from P. perfoliatum.