Objective:To observe the protective effect of ligustrazine and ferulate on myocardial ischemia-reperfusion injury and probe into its possible molecular mechanism in rats. Method:Sixty male rats were randomly divided into five groups:sham-operation group

ischemia-reperfusion group

ligustrazine(4 mg·kg-1) group

ligustrazine+ferulate low dose(4 mg·kg-1) group

ligustrazine+ferulate high dose(8 mg·kg-1) group.The rat model with ischemia-reperfusion injury was established with 30 min of myocardial ischemia and followed by 120 min reperfusion.The rats were treated for 10 min before reperfusion through jugular vein injection.After the reperfusion was finished

the biochemical indicators in serum and histological index in myocardium were investigated. Result:Compared with ischemia-reperfusion group

ligustrazine and ferulate could lower the level of creatine kinase isoenzyme(CK-MB)

lactate dehydrogenas(LDH)

cardiac troponin I(cTnI) and malondialdehyde(MDA) in blood

increase the activity of total-superoside dismutase(T-SOD) in blood

reduce the myocardial infarction size

increase the expression of Bcl-2 protein

decrease the expression of Bax protein

reduce the myocardial apoptosis index and Bcl-2/Bax ratio(P＜0.01);all indicators for ligustrazine and ferulate showed dose-dependently superior to ligustrazine(P＜0.05或P＜0.01). Conclusion:Ligustrazine and ferulate had a well protective effect on rats with myocardial ischemia-reperfusion injury.Anti-apoptotic effect was related to up-regulate the expression of Bcl-2 and down-regulate the expression of Bax by ligustrazine and ferulate.