Objective:To establish a high performance liquid chromatographic method (HPLC) for determination of baicalin in relieving itching cream intermediate products. Method: Agilent Eclipse Plus C18 column was used to separate the analyte with a mobile phase consisting of methyl alcohol and 0.37% phosphoric acid at a flow rate of 1 mL·min-1. The detection wavelength was set at 280 nm. Result: Baicalin showed good linearity (r2=0.999 9) in the range of the tested concentration (0.0792-0.792 μg); the average recovery of the method was 98.87% with RSD 1.14% (n=6). This method was used to successfully determine 3 batches relieving itching cream intermediate products

the content of baicalin in aqueous extract of Scutellaria Baicalensis was 5.75

6.08

5.94 g·L-1. Conclusion: This is a more stable

sensitive and precise method for monitoring relieving itching cream intermediate products

which could also be used as the quality control method for the process of the relieving itching cream.