Objective:To study dissolution regular pattern of ingredients from traditional Chinese medicine compound by solvent with different solubility parameter

and provide new ideas and theoretical basis for chromatographic pharmacodynamics sampling. Method: With different solubility parameters of solvent to dissolve Buyang Huanwu decoction extract

fingerprint chromatogram of ingredients from obtained lysate were determined by HPLC

and combining with total statistical moment theory

difference of solvent with different solubility parameter on component solution from total prescription was evaluated by total statistical moment similarity method. Result: RSD of AUCT

MCRTT

VCRTT from samples were 58.2%

12.8%

24.8%

respectively.It showed that chemical composition and total amount had significant difference

which was dissolved from solvent with different solubility parameter

its range of total statistical moment standard similarity 0.370-0.998.Sample similarity of S4

S5

S6 (solubility parameter of 18.33-20.60 J1/2·cm-3/2) compared to other samples

S7 and S8 (solubility parameter 21.73-22.87 J1/2·cm-3/2) compared to other samples except adjacent samples

these were less than 0.9

it indicated that constituent ratio of solvent on total prescription dissolution composition was different. Conclusion: Solubility parameter sampling method could make composition ratio of traditional Chinese medicine compound with significant difference

dissolution of solvent with different solubility parameter on total prescription had obvious law

it could provide an entirely new and referenced ideas for in vitro sampling with chromatographic pharmacodynamics

and could lay foundation for establishing a complete theoretical guidance of chromatographic pharmacodynamics sampling.