Objective: To study on effect of volatile oil from Amomum tsaoko on percutaneous penetration of Rotundine patch. Method: In vitro percutaneous penetration test in rat was conducted by using a modified Franz diffusion cell.Accumulative penetration volume of rotundine was determined by HPLC

enhancing effect of different concentrations of volatile oil from A. tsaoko on percutaneous permeation of rotundine in the patch was investigated. Result: The concentration of 5%

7% and 10% of volatile oil from A. tsaoko had a certain enhancing effect on permeation of rotundine in the patch

average cumulative penetration volume of rotundine in 12 h of 5%

7%

10% volatile oil were 3.848

4.329

4.914 mg·cm-2

multiples of enhancing permeation in 12 hours were 1.014

1.127

1.282. Conclusion: Volatile oil from A. tsaoko could enhance percutaneous penetration of rotundine in the patch.With a certain range

percutaneous penetration was enhancing with increasing the concentration of volatile oil from A. tsaoko.