Objective: To study the effect of Sophoridine on expression of super oxide dismutase (SOD)

malonaldehyde (MDA)and Toll-like receptor 4(TLR4 )of in acute lung injury (ALI ) mice induced by lipopolysaccharide(LPS). Method: Sixty healthy KM mice were randomly divided into 6 groups: normal group

model group

prevention treatment group

sophoridine high dose group

sophoridine middle dose group

low dose group sophoridine

10 mice each group. The normal group

model group

sophoridine high

middle and low groups were lavaged 0.5 mL sodium chloride and the prevention treatment group was lavaged 0.5 mL sophoridine every day. It lasted 6 days. The disposable intraperitoneal injection of endotoxin method was used to establish intends to induce acute lung injury model in mice. The model was set up in the sixth day by injecting LPS 9 mg·kg-1. 2 hours later

sophoridine high

middle and low groups were given 9

5

2.5 mg·kg-1 respectively. According to the manual of kits

SOD

MDA were detected. Reverse transcriptase-PCR was used to study the expression of TLR4 mRNA. Result: In model group SOD content (50.58±10.06) compared to other groups decreased significantly (P<0.05). In model group MDA content (2.01±0.15) and the expression of TLR4 mRNA compared to other groups increased significantly (P<0.05). Conclusion: Sophoridine can reduce ALI model mice lung tissue MDA content and the expression of TLR4 mRNA and increase the value of SOD.