Objective: To establish the methods of qualitation and quantitation about Trichosanthis Semen

one of the commonly used Chinese material medica. Method: The thin-layer chromatography (TLC) method was found with the reference substance both karounidiol and α-spinasterol. The HPLC method was found with the PAD and the reference substance of karounidiol. Result: The unsaponifiable matter from all 12 Trichosanthis Semen samples showed the spots of both karounidiol and α-spinasterol on a TLC plate. The content of karounidiol in all 12 Trichosanthis Semen samples was 0.056 88%-0.087 28%. Conclusion: Karounidiol

one of the active components of Trichosanthis Semen

can be considered as an important index of the quality standard of Trichosanthis Semen. The content of karounidiol was suggested not less than 0.05% in Trichosanthis Semen.