Objective:To investigate influence of chitosan flocculation sedimentation method on flavonoids and alkaloids from Gegen Qinlian decoction. Method: With puerarin

baicalin

berberine hydrochloride and extract rate as indexes

orthogonal design method was used to study effect of three factors including liquid concentrated degree

pH and the amount of chitosan on refining flavonoids and alkaloids from Gegen Qinlian decoction by chitosan flocculation sedimentation method

and compared with ethanol precipitation

centrifugal sedimentation and filtration method. Result: Optimum purification process of chitosan flocculation sedimentation method was:the mass concentration of liquid 0.78 g·mL-1

the addition amount of chitosan 10 %

pH 6

by comparing with different precipitation method

sedimentation effect of chitosan was the best. Conclusion: Chitosan could effectively retain active ingredients in liquid and reduce extract rate

but this method could change composition proportion of ingredients in liquid

so it should be cautious to choose this method.