Objective: To investigate the effects of centipede extraction on von willebrand disease fector(vWF) and thrombopoietin(TPO) expression after focal cerebral ischemia-reperfusion injury in rats. Method: Intraluminal thread occlusion of the middle cerebral artery(MCAO) was applied to establish the focal cerebral reperfusion model. The rats were randomly divided into six groups: sham-operative group

model group

high dose of centipede extraction group

middle dose of centipede extraction group

low dose of centipede extraction group and Yiqi Huoxue Recipe(YHR) group. The rats were reperfused again after occlusion of 2 hours. After intragastric administration of different drugs for 14 days

the blood plasma vWF and TPO were measured by enzyme-linked immunosorbent assay(ELISA). Result: The vWF and TPO levels were significantly up-regulated in rats after ischemia-reperfusion injury compared with sham-operative group. Compared with the model group

high dose of centipede extraction significantly ameliorated the vWF and TPO expression(P<0.05). Yiqi Huoxue Recipe administration could just decrease TPO level(P<0.05). The middle dose and low dose of centipede extraction reduced vWF and TPO levels without significant difference compared with model group. Conclusion: Centipede extraction could ameliorate cerebral ischemia-reperfusion symptoms via down-regulating the vWF and TPO levels.