HPLC测定双氢青蒿素软胶囊中双氢青蒿素的含量

詹利之; 詹爱琼; 林燕芳; 杨兆丽; 杨家庆; 张美义

您当前的位置：

首页 >

文章列表页 >

HPLC测定双氢青蒿素软胶囊中双氢青蒿素的含量

更新时间：2024-01-04

- HPLC测定双氢青蒿素软胶囊中双氢青蒿素的含量
- Determination of Dihydroartemisinin from Dihydroartemisinin Soft Capsule by HPLC
- 中国实验方剂学杂志 2012年18卷第19期页码：112-114
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  中图分类号：
- 纸质出版日期：2012
- 稿件说明：
移动端阅览
詹利之, 詹爱琼, 林燕芳, 等. HPLC测定双氢青蒿素软胶囊中双氢青蒿素的含量[J]. 中国实验方剂学杂志, 2012,18(19):112-114.

ZHAN Li-zhi, ZHAN Ai-qiong, LIN Yan-fang, et al. Determination of Dihydroartemisinin from Dihydroartemisinin Soft Capsule by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(19): 112-114.
詹利之, 詹爱琼, 林燕芳, 等. HPLC测定双氢青蒿素软胶囊中双氢青蒿素的含量[J]. 中国实验方剂学杂志, 2012,18(19):112-114. DOI：

ZHAN Li-zhi, ZHAN Ai-qiong, LIN Yan-fang, et al. Determination of Dihydroartemisinin from Dihydroartemisinin Soft Capsule by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(19): 112-114. DOI：

摘要

目的:建立双氢青蒿素软胶囊中双氢青蒿素含量测定的方法。方法:采用DikaDiamonsilC18柱(4.6mm×150mm

5μm);以0.1mol·L-1磷酸二氢钾溶液(pH2.5)-乙腈(65:35)为流动相;流速0.8mL·min-1;检测波长为234nm

柱温18℃。结果:双氢青蒿素在8~40μg·mL-1线性良好

r=0.99992

平均回收率99.6%

RSD0.66%。结论:该法操作简便、快捷、准确;适用于双氢青蒿素软胶囊中双氢青蒿素含量测定。

Abstract

Objective:To establish a method for the assay of dihydroartemisinin from Dihydroartemisinin Soft Capsules. Method:The assay was performed by a Diamonsil C18 columm(4.6 mm×150 mm

5 μm) and a mobile phase of 0.1 mol·L-1 potassium dihydrogen phosphate(pH 2.5)-acetonitrile with radio of 65:35

and the flow rate was 0.8 mL·min-1.The wavelength of detector was set at 234 nm and the column temperature was at 18℃. Result:Dihydroartemisinin has a good linearity in the range of 8-40 μg (r=0.999 92

n=5)

and the average recovery is 99.6% with RSD 0.7%(n=9). Conclusion:The method is simple

rapid and accurate and suitable for the determination of dihydroartemisinin in Dihydroartemisinin Soft Capsules.

关键词

Keywords

references

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

双氢青蒿素调控神经-小胶质细胞缓解神经病理性疼痛

双氢青蒿素对HepG2细胞氧化损伤和能量代谢的影响及其与索拉非尼的协同作用

毛冬青饮片指纹图谱及定量分析

双氢青蒿素对两种类风湿关节炎模型的影响及机制

铁皮石斛与霍山石斛中甘露糖、葡萄糖及柚皮素的含量比较