Objective: To observe the effect of Jinkui Shenqi Wan on the cardioventricular reconstruction in rat induced by Isoproterenol(ISO). Method: The rats were injected subcutaneously by 20

10

5 mg·kg-1 ISO for 3 days and then 3 mg·kg-1 ISO as maintenance dose for 7 days to build the rat model of cardioventricular reconstruction

Enalapril and Jinkui Shenqi Wan were given to the rats for 5 and 15 weeks as interventions and after that

stained the myocardium with Hematoxyln Eosin (HE) staining method and observed the myocardial morphologic changes by light microscope. The density of atrial natriuretic peptide (ANP)

brain natriuretic peptide (BNP)

norepinephrine (NE) and angiotensinⅡ (AngⅡ) in blood serum were detected by method of ELISA. Result: The results of neuroendocrine factors in blood serum: at 5 weeks

compared with model group

the density of ANP of Jinkui Shenqi Wan group was significantly lower (P<0.05). At weeks 15

compared with model group

the density of ANP and BNP of Jinkui Shenqi Wan group decreased (P<0.05). The density of NE in blood serum of model group at week 5 was higher than normal group

but with no significant difference

while that of model group at week 15 was significantly higher than normal group (P<0.05). At week 5 and 15

NE in the treat groups was no significant difference compared with model group. Both at weeks 5 and 15

there was not significant difference in the density of AngⅡin blood serum between model group and normal group. The results of pathomorphologic observation on myocardium: At week 5

the model group had many collagenoblast and neogenesis micrangium

while the collagenoblasts degenerated into fibrocytes

widespread fibrosis appearanced and the granulation tissues changed into scar tissues at week 15.Both at week 5 and 15

the myocardial cells in enalapril group and Jinkui Shenqi Wan have some extents of pathological changes

but it was less than model group. Conclusion: Jinkui Shenqi Wan has some therapeutic effects on cardioventricular reconstruction by suppressing the over-expression neuroendocrine factors both in earlier period and advanced stage of rat induced by isoproterenol. However

this effect is more obvious for advanced stage of cardioventricular reconstruction.