Objective: To establish the methods of determination on the contents of total flavonoids in Cardiospermum halicacabumby UV. Method: Rutin was used as reference substance and sodium nitrite-nitrate aluminum-sodium hydroxide as color-showing reagent

measurement wavelength was set at 488 nm. Result: The linear response range was 0.00935-0.0560 g·L-1(r=0.9993). The average recovery was 100.05%

RSD 2.3%(n=6)。 Conclusion: The method was simple

accurate and repeoducible which could be used for determination of the total flavonoids in C. halicacabum.