Objective: To establish the methods for determination of imperatorin and astragaloside in Tongqiaobiyan granule. Method: The HPLC method was performed on Diamonsil C18(4.6 mm×250 mm

5 μm)column;a mixture liquid of methanol-water(54∶46) was used as the mobile phase

and the detective wavelength was at 238 nm to determine the content of imperatorin. With Philo door 100A C18 (4.6 mm×150 mm

5 μm)column and evaporative light scattering detector

a mixture liquid of acetonitrile-water (32∶68)was used as the mobile phase to determine the content of astragaloside. Result: The calibration curve of imperatorin and astragaloside were linear in the ranges of 0.072 5-0.773 6

1.462 8-7.314 μg respectively. Conclusion: It is an accurate and credible quality control method.