Objective: To study the inhibition action of ethanol extract Toddalia asiatica in vitro against candida albicans growth and provide the theory basis for new drugs for the further development of the root canal disinfection. Method: Trace broth dilution method was used to determine the minimum inhibition concentration (MIC) and minimum bactericidal concentration (MBC) of the ethanol extract T. asiatica in vitro. Conventional scanning electron microscopy sample preparation was used to observe change of bacterial cells. The RT-PCR technology used to obtain complementary DNA

PCR amplification technology used to obtain SNF2 and PDE2 gene fragments of C.albicans virulence factors

products electrophoresis analysis was performed. Result: The MIC was 7.5 g·L-1

MBC was 15.0 g·L-1; scanning electron microscopy showed a irregular morphological changes of bacteria

the surface had obvious sag

crack; with the increased drug concentration

SNF2 and PDE2 expression was obviously declined. Conclusion: The ethanol extract T. asiatica on C. albicans has a good antibacterial action

which may be related to inhibiting expression of SNF2 and PDE2.