Objective: To study the prevention and treatment action of Shenge formula on CCl4 combined with high-fat and low-protein diet induced non-alcoholic fatty liver (NAFL) model. Method: The rat NAFL model was established by subcutaneous injection of carbon tetrachloride (CCl4) for 4 weeks twice per week combined with high-fat and low-protein diet for 2 weeks. All rats were randomly grouped into normal group

model group

Shenge formula group and positive drug group. Distilled water and corresponding drugs were administered by intragastric administration method every day. After rats were sacrificed

the serum aminotransferase value

liver pathological changes and triglyceride(TG)

free fatty acid(FFA) level

malondialdehyde(MDA)and superoxide dismutase (SOD) were examined. Result: Compared with model group

serum ALT and AST value were significantly decreased in Shenge formula group

and the MDA level in liver was significantly decreased in Shenge formula group(P<0.01). In addition

hepatic steatosis was significantly improved in Shenge formula. Conclusion: Shenge formula has protective effects on steatohepatitis

and it can significantly decrease the levels of TG and FFA

and improve inflammatory injury and hepatic steatosis

and anti-lipid peroxidation injury in the NAFL model.