Objective: To observe the influence of Sanqing Jiangtang Fang on α-glucosidase activity. Method: α-glucosidase from small intestine of rats was extracted and the content was determined. The optimal reaction system was set by orthogonal experiment. Sanqing Jiangtang Fang was extracted with water and ethanol respectively and prepared into solutions at 25

2.5

0.25

0.025

0.002 5 g·L-1. Acarbose was used as the positive drug. The content of glucose was measured by glucose oxidase method to evaluate the inhibitory effect on α-glucosidase. The inhibitory rate was calculated. Result: Water-extraction preparation and ethanol-extraction preparation of Sanqing Jiangtang Fang could inhibit α-glucosidase activity

and showed marked concentration-effect relationship. The inhibitory effect of water-extraction preparation at 25 g·L-1 was 64.85%±10.5%

which was in correspondence to acarbose at 2.5 g·L-1. Conclusion: Sanqing Jiangtang Fang can inhibit α-glucosidase activity.