DONG Jie, ZHU Hui-min, HU Kai-wen, et al. Optimization of Purification Process for Total Alkaloids from by Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(14): 18-22.
DONG Jie, ZHU Hui-min, HU Kai-wen, et al. Optimization of Purification Process for Total Alkaloids from by Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(14): 18-22.DOI:
Objective: To optimize purification process of total alkaloids from Fritillaria thunbergii by macroporous resin. Method: Optimum macroporous resin and its purification process was selected by static and dynamic adsorption and desorption kinetics test. Result: Optimal purification process conditions were:optimal macroporous resin type HPD100
diameter-height ratio 1∶9
the concentration of sample solution 5 g·mL-1 with pH as 8.0
maximum sample volume 13 BV
flow rate of adsorption and elution 1 mL·min-1
washed by 8 BV water and 10 BV 80% ethanol
collected eluent of 80% ethanol.Under this optimized technology
the content of total alkaloids was more than 65% by UV. Conclusion: HPD100 type macroporous resin showed good comprehensive adsorption property for enrichment of total alkaloids from F. thunbergii
and suitable for separation and purification of it.