一测多评法与外标法测定新清宁片中大黄蒽醌类成分含量

张锴镔; 冯伟红; 王智民; 张启伟; 李霞; 杨菲; 李东影; 吉丽娜

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一测多评法与外标法测定新清宁片中大黄蒽醌类成分含量

更新时间：2024-01-04

- 一测多评法与外标法测定新清宁片中大黄蒽醌类成分含量
- Determination of Rhubarb Anthraquinones in Tablets by QAMS and External Standard Method
- 中国实验方剂学杂志 2012年18卷第11期页码：61-65
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  2007年中医药行业科技专业项目(200707009),国家"重大新药创制"科技重大专项(2009ZX09301-005,2009ZX09308-003)
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- 纸质出版日期：2012
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张锴镔, 冯伟红, 王智民, 等. 一测多评法与外标法测定新清宁片中大黄蒽醌类成分含量[J]. 中国实验方剂学杂志, 2012,18(11):61-65.

ZHANG Kai-bing, FENG Wei-hong, WANG Zhi-min, et al. Determination of Rhubarb Anthraquinones in Tablets by QAMS and External Standard Method[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(11): 61-65.
张锴镔, 冯伟红, 王智民, 等. 一测多评法与外标法测定新清宁片中大黄蒽醌类成分含量[J]. 中国实验方剂学杂志, 2012,18(11):61-65. DOI：

ZHANG Kai-bing, FENG Wei-hong, WANG Zhi-min, et al. Determination of Rhubarb Anthraquinones in Tablets by QAMS and External Standard Method[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(11): 61-65. DOI：

摘要

目的: 采用"一测多评"法与外标法测定新清宁片中4种大黄蒽醌类成分的含量

并对测定结果进行比较。方法: 采用RP-HPLC

Accurasil C18色谱柱(4.6 mm×250 mm

5 μm)

流动相甲醇-0.4%磷酸水溶液(85 :15)

流速1 mL·min-1

柱温30 ℃

检测波长254 nm。结果: 20批新清宁片中4种蒽醌类成分QAMS计算值与ESM实测值无显著性差异

RSD<5%。结论: 在一定色谱条件下

QAMS确定的相对校正因子可作为常数用于含大黄制剂的质量评价。

Abstract

Objective: To determine the contents of four kinds of anthraquinone components in Xinqingning tablet by quantitative analysis of multi-component with a single-marker(QAMS) and external standard method(ESM) respectively

and compare results by this two methods. Method: RP-HPLC determination was performed at 30 ℃ with Accurasil C18 column (4.6 mm×250 mm

5 μm)

mobile phase of methanol-0.4% phosphoric acid (85 : 15)

detection wavelength at 254 nm

and flow rate of 1.0 mL·min-1. Result: There was no significant difference between calculated value of QAMS and measured value of ESM for four kinds of anthraquinone components from Xinqingning tablet

RSD<5%. Conclusion: Under certain chromatographic conditions

relative correlation factor could be used as a constant for quality evaluation of Rheum palmatum preparation which was determined by QAMS.

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