Objective: To research the chromatography fingerprint of Radix Astragali with hyphenated technique of HPLC-DAD-ELSD. Method: The chromatography conditions were defined as SpursilTM C18 column (4.6 mm× 250 mm

5 μm); mobile phase was A:ACN

B:H2O

gradient elution; flow speed was 1.0 mL·min-1; temperature of column was set at 25 ℃; injection volume was 20 μL; detected wavelength was at 254 nm. The ELSD conditions were as follows: the temperature of drift tube was 112.8 ℃

the gas speed was 3.2 L·min-1. Result: In the chromatography fingerprint with HPLC-DAD of Radix Astragali

14 common peaks were demarcated

calycosin and formononetin were identified; in the chromatography fingerprint with HPLC-ELSD of Radix Astragali

9 common peaks were demarcated

calycosin-7-O-β-D-glucoside

astragaloside Ⅳ

astragaloside Ⅲ and astragaloside Ⅱ were identified. Conclusion: The method was accurate and reliable and the study may contribute in controlling of Radix Astragali.