Objective: To establish a method for Dioscorea nipponica rats serum HPLC fingerprinting. Method: The separation was performed on a Diamonsil C18 column (4.6 mm×250 mm

5 μm) with 0.1% phosphoric acid water and acetonitrile as mobile phase by gradient elution; the flow rate was 0.8 mL·min-1; detection wavelength was set at 254 nm; the column temperature was maintained at 25 ℃. Result: The RSD of precision

reproducibility

stability test of relative peak area and relative retention time was less than 5%. Conclusion: The method is simple

practical

reliable

which would lay a solid foundation for further studies on pharmacodynamic material basis of Dioscorea nipponica and pharmacodynamic action mechanism research.