SONG Zhi-zhao, LIU Yuan. Determination of Quercitrin in by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(13): 99-101.
SONG Zhi-zhao, LIU Yuan. Determination of Quercitrin in by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(13): 99-101.DOI:
Objective: To assay the content of quercitr in Gaultheriae leucocarpa by HPLC. Method: The Appollo-C18 (4.6 mm×250 mm
5 μm) column was used
the mobile phase consisted of acetonitrile-0.1%phosphoric acid (23: 77)
the detection wave length was at 254 nm and the column temperature was at room temperature. Result: Linearity of quercitr was good at 0.027 5-0.88 μg (r=1.00). The average recovery of buddleodide was 102.7% with RSD less than 1.65%(n=6). Conclusion: The method is simple
accurate and repeatable
which can be applied in determination of quercitrin in G. leucocarpa.