黄连解毒汤抗铜绿假单胞菌生物被膜 及与阿奇霉素协同抗菌作用

朱小明; 杨家卿; 张昌峰; 程惠娟

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黄连解毒汤抗铜绿假单胞菌生物被膜及与阿奇霉素协同抗菌作用

更新时间：2024-01-04

- 黄连解毒汤抗铜绿假单胞菌生物被膜及与阿奇霉素协同抗菌作用
- The Effects of Huanglian Jiedu Decoction against Biofilms and its Synergism with Azithromycin on Planktonic
- 中国实验方剂学杂志 2012年18卷第11期页码：155-158
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金面上项目(81173629)
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  中图分类号：
- 纸质出版日期：2012
- 稿件说明：
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朱小明, 杨家卿, 张昌峰, 等. 黄连解毒汤抗铜绿假单胞菌生物被膜及与阿奇霉素协同抗菌作用[J]. 中国实验方剂学杂志, 2012,18(11):155-158.

ZHU Xiao-ming, YANG Jia-qin, ZHANG Chang-feng, et al. The Effects of Huanglian Jiedu Decoction against Biofilms and its Synergism with Azithromycin on Planktonic [J]. Chinese journal of experimental traditional medical formulae, 2012, 18(11): 155-158.
朱小明, 杨家卿, 张昌峰, 等. 黄连解毒汤抗铜绿假单胞菌生物被膜及与阿奇霉素协同抗菌作用[J]. 中国实验方剂学杂志, 2012,18(11):155-158. DOI：

ZHU Xiao-ming, YANG Jia-qin, ZHANG Chang-feng, et al. The Effects of Huanglian Jiedu Decoction against Biofilms and its Synergism with Azithromycin on Planktonic [J]. Chinese journal of experimental traditional medical formulae, 2012, 18(11): 155-158. DOI：

摘要

目的: 研究黄连解毒汤抗铜绿假单胞菌生物被膜及与阿奇霉素协同抗菌作用。方法: 微量倍比稀释法测定黄连解毒汤对铜绿假单胞菌的最小抑菌浓度(MIC)

棋盘稀释法测定黄连解毒汤和阿奇霉素协同抗菌作用

MTT法测定黄连解毒汤对铜绿假单胞菌生物被膜的最小抑膜浓度(SMIC)

显微镜下观察药物对生物膜形态的影响。结果: 黄连解毒汤对铜绿假单胞菌的MIC 100 g·L-1

阿奇霉素200 mg·L-1

两药联合后MIC 分别为25 g·L-1和25 mg·L-1抑菌分级指数 (FIC)0.187 5

提示两药协同作用明显。黄连解毒汤对铜绿假单胞菌生物被膜的SMIC50 1

3 d均为15.6 g·L-1

7 d 31.25 g·L-1;SMIC801

7 d均为250 g·L-1

形态观察提示黄连解毒汤SMIC80浓度对铜绿假单胞菌生物被膜的抑制作用明显。结论: 黄连解毒汤具有抗铜绿假单胞菌生物被膜作用

与阿奇霉素有协同抗菌作用。

Abstract

Objective: To investigate the effects of Huanglian Jiedu decoction against Pseudomonas aeruginosa biofilms and its synergistic antibacterial in combination with azithromycin. Method: The microdilution assay was used to detect MIC of Huanglian Jiedu decoction against P. aeruginosa

checkerboard method was used to examine synersism of Huanglian Jiedu decoction and azithromycin. MTT assay was used to detect SMIC of Huanglian Jiedu decoction against P. aeruginosa biofilms

and microscopes was used to observe morphology of biofilms treated with the two drugs. Result: MIC of Huanglian Jiedu decoction against P. aeruginosa was 100 g·L-1and azithromycin 200 mg·L-1. After the combination of two-drug MIC was 25 g·L-1 and 25 mg·L-1fractional inhibitory conceritration(FIC) 0.1875

which showed a synergismwith azithromycin.SMIC50 of Huanglian Jiedu Decoction against P. aeruginosa biofilms at 1

7 d was 15.6

15.6

31.25 g·L-1

respectively; SMIC80 of the biofilms was 250

250

250 g·L-1

respectively. Morphologically Huanglian Jiedu decoction inhibited P. aeruginosa biofilms evidently at the concentration of SMIC80. Conclusion: Huanglian Jiedu decoction could inhibit P. aeruginosa biofilms

and has a synergism with azithromycin.

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