Objective: To determine the effective part of anti-hyperuricemia in Gardeniae Fructus. Method: Four fractions were obtained by systematic solvent extraction

petrol ether extract

ethyl acetate extract

butanol extract and water-soluble part.Total extract of Gardenia Fructus (TEG) and its different extracts were administrated to hyperuricemia mice induced by potassium oxonate

then the level of uric acid and xanthine oxidase in hyperuricemia mice were observed. Result: TEG

ethyl acetate extract and water-soluble part could obviously reduce the level of serum uric acid in hyperuricemia mice

TEG

petrol ether extract and water-soluble part could obviously inhibit the activity of and xanthine oxidase in the liver. TEG and its different extracts had no effect on the level of serum uric acid in normal mice. Conclusion: Ethyl acetate extract and water-soluble part from Gardeniae Fructus are the active fraction for anti-hyperuricemia

and the effect is related to its inhibition of xanthine oxidase.