Objective:To observe the effect of the antioxidation on Parbin II formula in Parkinson's disease (PD) rats. Method: PD rats were induced by injection of 6-hydroxydopamine (6-OHDA) twice stereotaxically into the left side of striatum

the rotation test was diagnosed by injecting apomorphin (APO)

the PD rats were randomly divided into five groups: model group

Parbin Ⅱ formula (32.0

16.0

8.0 g·kg-1) and madopar group(0.075 g·kg-1)

at the same time

the normal group was established and the treatment lasted for 4 weeks. The model group and the normal group were given vehicle only. The colorimetric assays were used to detect the activities of glutathione peroxidase(GSH-Px)

superoxidase dismutase (SOD) and the levels of malonaldehyde (MDA) in tissue homogenate that came from the the left side of striatum and behavioural changes were detected by injection of APO after the treatment. Result: There was no significant difference in the rotation behavior between before and after treatment in model group; compared with model group

the rotation behavior was significantly decreased in Parbin Ⅱ formula high and media dose group(P<0.05)

and there was a significant difference between before and after treatment (P<0.05). Madopar could also change the rotation behavior in PD rats. However

Parbin Ⅱ formula low dose group had no change. Compared with the normal control group

the contents of MDA were obviously increased and the activities of GSH-Px and SOD were obviously reduced in model group (P<0.05). However

the above indicators were improved in Parbin Ⅱ Formula treatment group

the high

media dose group can dereased obviously MDA contents and had the significant difference (P<0.05)

which were (9.29±3.25)

(9.62±2.78) nmol·mg-1 respectively

increased obviously the activities of GSH-Px and SOD (P<0.05)

which was (612.53±53.20)

(637.03±133.21) NU·g-1

(123.93±9.84)

(120.21±10.63) U·mg-1 respectively; low dose group could only remove the activities of GSH-Px and had no change in MDA and SOD contents. However

there was no significant difference in the above indicators in madopar group. Conclusions: Pabing Ⅱ formula can significantly improve the rotation behavior of PD model rats

enhance antioxidation ability and eliminate free radicals ability

and its effect is in a dose dependent manner.