Objective: To observe the mechanism of antidiarrheal effect of Yunpi Zhixie formulas (smoothing spleen to stop diarrhea formulas). Method: Rats were randomly divided into three groups: control group (distilled water)

drug (Yunpi Zhixie formulas)group and model group(reserpine). Reserpine was administrated to rats of drug and model group by intraperitoneal injection for 3 days in a prophylactic manner. In consequent 6 days

distilled water was administrated to model group

while Yunpi Zhixie formulas were administrated to drug group. The changes in D-xylose

pepsin

motilitin(MTL)

vasoactive intestinal peptide(Vip)

acetylcholinesterase(AChE) and microvilli were observed. Result: After reserpine was administrated

the level of the D-xylose

pepsin

AChE and Vip decreased

MTL increased. Microvilli were disturbed severely. After Yunpi Zhixie formulas was administrated

the level of D-xylose·L-1] is higher than those in model and control group·L-1].Pepsin·mg-1] is higher than that in model group·mg-1] obviously

MTL·mg-1] was lower than that in model group·mg-1]. Vip·L-1] was back to control group's level·L-1]. AChE·mg-1] was higher than model group·mg-1]

but still lower than that in control group·mg-1].The microvilli were almostly repaired. Conclusion: Yunpi Zhixie formulas by Wang Peng-fei under the direction of warming middle to invigorate spleen inhibits diarrhea through promoting digestion

absorption

slowing motility of gastrointestinal tract and provides a protection for microvilli.